Skip to main content

Table 2 Glutamine controls recHCG glycoforms in bioreactor continuous culture.

From: Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

Peak

No.

Glycan Structure

Steady State 1

(8 mM Gln)

Steady State 2

(0 mM Gln)

Steady State 3

(8 mM Gln)

Steady State 4

(0 mM Gln)

1

A1

2.16 ± 0.66

4.57 ± 2.64

4.58 ± 2.05

4.44 ± 1.34

2

A2

1.13 ± 0.27

1.30 ± 0.21

2.31 ± 0.50

1.36 ± 0.24

3

A1G1

1.32 ± 0.24

1.90 ± 0.40

2.64 ± 0.60

5.89 ± 0.97

4

M5

4.56 ± 0.44

4.72 ± 0.61

6.77 ± 1.01

14.70 ± 3.37

5

A2G1, A1G1S1

3.60 ± 0.19

3.45 ± 0.06

3.81 ± 0.35

4.66 ± 0.81

6

A2G1S1

1.42 ± 0.19

1.63 ± 0.24

1.65 ± 0.22

2.41 ± 0.22

7

FA2G1

3.53 ± 0.47

3.54 ± 0.23

3.78 ± 0.42

2.40 ± 0.27

8

A2G2, FA2G1S1

7.39 ± 0.59

8.38 ± 0.91

9.29 ± 1.31

14.33 ± 2.08

9

FA2G2, A2G2S1

18.31 ± 0.98

18.03 ± 0.84

16.56 ± 0.36

22.06 ± 2.92

10

A2G2S2, FA2G2S1

26.90 ± 0.73

24.51 ± 1.04

20.90 ± 2.18

14.55 ± 1.15

11

A3G2S1, FA2G2S2

20.99 ± 1.60

20.14 ± 1.42

16.02 ± 2.29

8.87 ± 0.84

12

A3G2S2, A3G3S1

2.95 ± 0.23

2.79 ± 0.26

5.54 ± 1.28

2.23 ± 0.18

13

FA3G3S1, A3G3S2

2.67 ± 0.25

2.40 ± 0.19

3.77 ± 0.62

1.23 ± 0.17

14

A3G3S3, FA3G3S2

1.97 ± 0.11

1.70 ± 0.15

1.50 ± 0.29

0.57 ± 0.06

15

FA3G3S3

1.07 ± 0.14

0.96 ± 0.13

0.90 ± 0.14

0.30 ± 0.04

  1. The relative structures and proportions of each peak of the HCG N-linked glycan NP-HPLC pool with varying concentrations of glutamine at each steady state of continuous culture are shown. Numbers in bold indicates significant difference, where each steady state was compared side-by-side, p < 0.05 and n = 3.