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Table 2 Glutamine controls recHCG glycoforms in bioreactor continuous culture.

From: Synergizing metabolic flux analysis and nucleotide sugar metabolism to understand the control of glycosylation of recombinant protein in CHO cells

Peak
No.
Glycan Structure Steady State 1
(8 mM Gln)
Steady State 2
(0 mM Gln)
Steady State 3
(8 mM Gln)
Steady State 4
(0 mM Gln)
1 A1 2.16 ± 0.66 4.57 ± 2.64 4.58 ± 2.05 4.44 ± 1.34
2 A2 1.13 ± 0.27 1.30 ± 0.21 2.31 ± 0.50 1.36 ± 0.24
3 A1G1 1.32 ± 0.24 1.90 ± 0.40 2.64 ± 0.60 5.89 ± 0.97
4 M5 4.56 ± 0.44 4.72 ± 0.61 6.77 ± 1.01 14.70 ± 3.37
5 A2G1, A1G1S1 3.60 ± 0.19 3.45 ± 0.06 3.81 ± 0.35 4.66 ± 0.81
6 A2G1S1 1.42 ± 0.19 1.63 ± 0.24 1.65 ± 0.22 2.41 ± 0.22
7 FA2G1 3.53 ± 0.47 3.54 ± 0.23 3.78 ± 0.42 2.40 ± 0.27
8 A2G2, FA2G1S1 7.39 ± 0.59 8.38 ± 0.91 9.29 ± 1.31 14.33 ± 2.08
9 FA2G2, A2G2S1 18.31 ± 0.98 18.03 ± 0.84 16.56 ± 0.36 22.06 ± 2.92
10 A2G2S2, FA2G2S1 26.90 ± 0.73 24.51 ± 1.04 20.90 ± 2.18 14.55 ± 1.15
11 A3G2S1, FA2G2S2 20.99 ± 1.60 20.14 ± 1.42 16.02 ± 2.29 8.87 ± 0.84
12 A3G2S2, A3G3S1 2.95 ± 0.23 2.79 ± 0.26 5.54 ± 1.28 2.23 ± 0.18
13 FA3G3S1, A3G3S2 2.67 ± 0.25 2.40 ± 0.19 3.77 ± 0.62 1.23 ± 0.17
14 A3G3S3, FA3G3S2 1.97 ± 0.11 1.70 ± 0.15 1.50 ± 0.29 0.57 ± 0.06
15 FA3G3S3 1.07 ± 0.14 0.96 ± 0.13 0.90 ± 0.14 0.30 ± 0.04
  1. The relative structures and proportions of each peak of the HCG N-linked glycan NP-HPLC pool with varying concentrations of glutamine at each steady state of continuous culture are shown. Numbers in bold indicates significant difference, where each steady state was compared side-by-side, p < 0.05 and n = 3.