Table 2 Primer sets and conditions used in quantitative real-time PCR (qPCR)
Gene | Genbank Accession | Sense primer 5'----- > 3' (Tm°C) | Antisense primer 3'----- > 5' (Tm°C) | Size amplicon (bp) | Annealing temp. (°C) | Putative function |
|---|---|---|---|---|---|---|
ALB | NM_000477 | TGAGAAAACGCCAGTAAGTGAC (60.8) | TGCGAAATCATCCATAACAGC (58.7) | 265 | 60 | albumin |
AFP | NM_001134 | GCTTGGTGGTGGATGAAACA (60.4) | TCCTCTGTTATTTGTGGCTTTTG (59.2) | 157 | 60 | α-fetoprotein |
CK18 | X12881 | GAGATCGAGGCTCTCAAGGA (62.4) | CAAGCTGGCCTTCAGATTTC (60.4) | 357 | 60 | cytokeration 18 |
G6PD | U01120 | GCTGGAGTCCTGTCAGGCATTGC (68.1) | TAGAGCTGAGGCGGAATGGGAG (66.4) | 349 | 60 | glucose-6-phosphate dehydrogenase |
HNF-4α | AY680696 | GCCTACCTCAAAGCCATCAT (60.4) | GACCCTCCCAGCAGCATCTC (66.5) | 256 | 60 | hepatocyte nuclear factor 4α |
TAT | NM_000353 | TGAGCAGTCTGTCCACTGCCT (64.5) | ATGTGAATGAGGAGGATCTGAG (60.8) | 338 | 60 | tyrosine aminotransferase |
CYP2B6 | NM_000767 | ATGGGGCACTGAAAAAGACTGA (60.8) | AGAGGCGGGGACACTGAATGAC (66.4) | 283 | 60 | Cytochrome P450 2B6 |
CYP2D6 | NM_000106 | CTAAGGGAACGACACTCATCAC (62.7) | GTCACCAGGAAAGCAAAGACAC (62.7) | 289 | 60 | Cytochrome P450 2D6 |
CYP2C9 | NM_000771 | CCTCTGGGGCATTATCCATC (62.4) | ATATTTGCACAGTGAAACATAGGA (57.7) | 137 | 60 | Cytochrome P450 2C9 |
CYP2C19 | NM_000769 | TTCATGCCTTTCTCAGCAGG (60.4) | ACAGATAGTGAAATTTGGAC (54.3) | 277 | 60 | Cytochrome P450 2C19 |
CYP2C8 | NM_000770 | ACAACAAGCACCACTCTGAGATATG | GTCTGCCAATTACATGATCAATCTCT | 100 | 60 | Cytochrome P450 2C8 |
CYP3A4 | AK298451 | GCCTGGTGCTCCTCTATCTA (62.4) | GGCTGTTGACCATCATAAAAGC (60.8) | 187 | 60 | Cytochrome P450 3A4 |
CYP1A1 | BC023019 | TCCAGAGACAACAGGTAAAACA (58.9) | AGGAAGGGCAGAGGAATGTGAT (62.7) | 371 | 60 | Cytochrome P450 1A1 |
CYP1A2 | AF182274 | ACCCCAGCTGCCCTACTTG (64.5) | GCGTTGTGTCCCTTGTTGT (62.4) | 101 | 60 | Cytochrome P450 1A2 |
CYP2E1 | NM_000773 | ACCTGCCCCATGAAGCAACC (64.5) | GAAACAACTCCATGCGAGCC (62.4) | 246 | 60 | Cytochrome P450 2E1 |
PXR | AB307701 | GAAGTCGGAGGTCCCCAAA (62.3) | CTCCTGAAAAAGCCCTTGCA (60.4) | 100 | 60 | pregnane × receptor |
CAR | AB307702 | TGATCAGCTGCAAGAGGAGA (60.4) | AGGCCTAGCAACTTCGCACA (62.4) | 102 | 60 | constitutive androstane receptor |
AhR | BC070080 | ACATCACCTACGCCAGTCGC (64.5) | TCTATGCCGCTTGGAAGGAT (60.4) | 101 | 60 | aryl hydrocarbon receptor |
UGT1A1 | BC128414 | GGAGCAAAAGGCGCCATGGC (62.5) | GTCCCCTCTGCTGCAGCTGC (64.5) | 178 | 60 | uridine diphosphate glucuronyltransferase 1A1 |
LV-BMI-1 | NM_005180 | GCTGAGGGCTATTGAGGCGCA (65.5) | ACCCCAAATCCCCAGGAGCTGT (65.7) | 127 | 60 | lentivirus vector BMI-1 |
hBMI-1 | NM_005180 | ACCTCCCAGCCCCGCAGAAT (65.9) | AGACGCCGCTGTCAATGGGC (66.4) | 280 | 60 | human BMI-1 |
LV-hTERT | AF018167 | CAACCCGGCACTGCCCTCAG (66.9) | GGGGTTCCGCTGCCTGCAAA (68.2) | 268 | 60 | lentivirus vector telomerase reverse transcriptase |
hTERT | AF018167 | CGGAAGAGTGTCTGGAGCAAGT (59.5) | GAACAGTGCCTTCACCCTCGA (61.1) | 258 | 60 | human telomerase reverse transcriptase |