Table 1 Bioassay showing larvicidal activity including Cry8Ka1 toxin, the others toxins encoded by the seven selected gene variants and VCSM13 Phage (as negative control) using A. grandis larvae.

VCSM13 Phage (Control)

6

20.55 ± 2.50 b¹

1.02

Cry8Ka1 (Wild-type)

5

29.33 ± 5.47 a,b

2.44

Cry8Ka2

5

36.66 ± 5.77 a,b

2.58

Cry8Ka3

5

49.33 ± 6.30 a

2.81

Cry8Ka4

6

33.88 ± 11.95 a,b

4.88

Cry8Ka5

4

50.00 ± 11.54 a

5.77

Cry8Ka6

6

32.77 ± 15.22 a,b

6.21

Cry8Ka7

6

44.44 ± 19.16 a,b

7.82

Cry8Ka8

5

45.99 ± 17.50 a,b

7.82