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Table 1 Bioassay showing larvicidal activity including Cry8Ka1 toxin, the others toxins encoded by the seven selected gene variants and VCSM13 Phage (as negative control) using A. grandis larvae.

From: Improving Cry8Ka toxin activity towards the cotton boll weevil (Anthonomus grandis)

Treatment N Lethality Mean (%) ± SD SEM
VCSM13 Phage (Control) 6 20.55 ± 2.50 b1 1.02
Cry8Ka1 (Wild-type) 5 29.33 ± 5.47 a,b 2.44
Cry8Ka2 5 36.66 ± 5.77 a,b 2.58
Cry8Ka3 5 49.33 ± 6.30 a 2.81
Cry8Ka4 6 33.88 ± 11.95 a,b 4.88
Cry8Ka5 4 50.00 ± 11.54 a 5.77
Cry8Ka6 6 32.77 ± 15.22 a,b 6.21
Cry8Ka7 6 44.44 ± 19.16 a,b 7.82
Cry8Ka8 5 45.99 ± 17.50 a,b 7.82
  1. 10 μg/ml of expressed phage were used in every treatment
  2. N, number of bioassays (12 larvae/replicate); SD, standard deviation; SEM, Standard Error Mean.
  3. 1 Results indicated with the same letter have no statistical difference between treatments (p < 0,05).