Ribbon diagrams of the Cry8Ka1 structural model produced with PyMOL and indicating the positions of the amino acid substitutions obtained in Cry8Ka5 toxin. A: The whole molecule showing the three domains (Domain I in brown color, II in blue color and III in yellow color). Cry8Ka5 substituted residues are highlighted in magenta color. In B, Domain I, composed of eight α-helices, is shown. The position of the R82Q mutation is indicated on helix α3 (also indicated in Fig 7). In C, the anti-parallel β-sheets of domain II, indicating two substituted residues in Cry8Ka5 toxin: Cys and Ala in positions 260 and 321, respectively. In D, the jelly-roll β-sheets of Domain III, and the three other mutated residues: Gly at position 508, Glu at 538 and Asn at 594.