Figure 2

Biopanning screening of the cry8ka1 Phage display combinatorial library for interactions of Cry8Ka1variants with Brush Border Midgut Vesicles (BBMVs) from cotton boll weevil larvae ( A. grandis ). The absorbance indicated in the graph shows the phage-infected, which was related to the quantity of bacterial colonies obtained from each round of biopanning. Based in the data, the fifth round of biopanning was chosen as cycle of the enrichment of recombinant phages displaying Cry8Ka1 variants specifically bound to A. grandis BBMVs. The points of the curve indicate the absorbance at 405 nm (reading of phage) in each round.