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Table 2 Free amino acid uptake and release during HEK293SF cell growth after infection.

From: Metabolic and Kinetic analyses of influenza production in perfusion HEK293 cell culture

   Gln Asp Glu Ser Asn Gly His Thr Arg Ala Pro Cys Val Met Ile Leu Lys Phe
Free amino acid level before cell infection, mM Batch 2.21 1.10 1.62 5.84 7.95 0.66 0.98 2.87 1.78 1.01 4.36 0.43 2.50 0.80 2.31 3.57 2.66 1.14
  Perfusion 1.77 0.87 1.48 2.80 7.06 0.73 0.87 2.17 1.32 1.28 3.70 0.02 2.04 0.67 1.91 3.11 2.17 0.96
Specific uptake or release of free amino acids, μmol.10-6 cell Batch 0.09 0.00 -0.03 0.01 -0.06 -0.03 0.01 -0.05 0.01 -0.09 0.05 -0.04 0.03 0.01 0.01 -0.01 -0.03 -0.01
  Perfusion 0.12 0.02 -0.01 0.08 0.00 -0.06 0.01 0.03 0.04 -0.08 0.00 0.02 0.03 0.01 0.05 0.09 0.06 0.01
  1. Values for specific uptake and release were calculated between infection time and the start of cell death (24 h post-infection). Positive values represent amino acid uptake, while negative ones represent their release.