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Table 2 Maximal biomass and glutathione (TIG and TEG) production of H. polymorph a strains at different conditions of fermentation

From: Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorphaDL-1

Strains Conditions of fermentation TIG TEG Biomass
     nmol/mg protein % w/w mg/g of dry cells mg/L mg/L Dry cells g/L
Wild-types NCYC495L     177 ± 1.5 0.6 6 20 ± 0.15 1 ± 0.05 3.2 ± 0.26
   CBS 4732L Shake- Glucose 200 rpm (w/o pH control) 380 ± 3.2 0.9 9 24 ± 0.19 1 ± 0.06 2.8 ± 0.19
   DL-1L flask    481 ± 3.9 1.0 10 27 ± 0.21 2 ± 0.09 2.8 ± 0.18
    200 rpm (pH control 5.2) 161 ± 1.49 0.5 5 20 ± 1.4 2 ± 0.19 4.0 ± 0.36
  Batch   600 rpm (- " - " - " - " - ) 50 ± 0.45 0.3 3 17 ± 1.19 12 ± 1.10 6.9 ± 0.42
Wild type DL-1 Fermenter Glucose 200 rpm (w/o pH control) 373 ± 3.12 1.1 11 69 ± 6.49 2 ± 0.20 6.1 ± 0.39
    800 rpm (- " - " - " - " - ) 269 ± 2.11 0.6 6 49 ± 4.45 17 ± 1.59 7.7 ± 0.45
Wild type DL-1   Methanol DO-stat 60% 136 0.8 8 676 37 90
Wild type DL-1   Glucose DO- stat 60% 183 1.2 12 518 22 44
Wild type DL-1   Glucose DO- stat 30% 315 1.3 13 910 4 72
mcMET4 Fed-batch Glucose (- " - " - " - " ) 318 1.6 16 1318 6 82
mcGSH2 fermenter Glucose (- " - " - " - " ) 755 2.0 20 1532 1 77
MOXp-GSH2   Glucose (- " - " - " - " ) 409 3.1 31 2257 13 72
MOXp-GSH2   Methanol DO-stat 60% 243 2.1 21 1053 250 55