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Figure 3 | BMC Biotechnology

Figure 3

From: Optimization of glutathione production in batch and fed-batch cultures by the wild-type and recombinant strains of the methylotrophic yeast Hansenula polymorphaDL-1

Figure 3

Southern blot analysis of copy number of HpGSH2 and HpMET4 genes in mcGSH2 and mcMET4 transformants. Copy number of integrated into chromosomal DNA of H. polymorpha DL-1L wild type strain (1), mcGSH2 (2) and mcMET4 (3) transformants pGLG61-HpGSH2 and pGLG61-HpMET4 plasmids bearing LEU2 gene. The DNA probes to LEU2 gene discovered mutated LEU2 gene (a) in all tested strains and introduced LEU2 gene based on indicated plasmids only in transformants (b).

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BMC Biotechnology

ISSN: 1472-6750

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