Figure 7
From: Site-specific modification of ED-B-targeting antibody using intein-fusion technology
L19 IgG-DnaE N after PTS. Analysis by reducing SDS-PAGE (Coomassie stained). Purified fusion protein was treated with 5 mM DTT and 50 μM DnaEC peptide for 22h at RT. After dialysis, trans-spliced L19 IgG was purified by His tag affinity chromatography (1: L19 IgG-DnaEN before PTS, 2: after PTS, before purification, 3: after purification, HC: heavy chain, LC: light chain, * product not further investigated).