Table 1 MiRNAs, PCR primers and synthetic templates
From: Specific and sensitive quantitative RT-PCR of miRNAs with DNA primers
miRNA | Sequence | Forward primer | Reverse primer | Synthetic template |
|---|---|---|---|---|
let-7a | UGAGGUAGUAGGUUGUAUAGUU | GCAGTGAGGTAGTAGGTTGT | GGTCCAGTTTTTTTTTTTTTTTAACTATAC | CAGGTCCAGTTTTTTTTTTTTTTTAACTATACAACCTACTACCTCA |
let-7d | AGAGGUAGUAGGUUGCAUAGUU | AGAGAGGTAGTAGGTTGCAT | AGGTCCAGTTTTTTTTTTTTTTTAACT | CAGGTCCAGTTTTTTTTTTTTTTTAACTATGCAACCTACTACCTCT |
miR-20a | UAAAGUGCUUAUAGUGCAGGUAG | ACAGTAAAGTGCTTATAGTGCA | GTCCAGTTTTTTTTTTTTTTTCTACCT | CAGGTCCAGTTTTTTTTTTTTTTTCTACCTGCACTATAAGCACTTTA |
miR-21 | UAGCUUAUCAGACUGAUGUUGA | TCAGTAGCTTATCAGACTGATG | CGTCCAGTTTTTTTTTTTTTTTCAAC | CAGGTCCAGTTTTTTTTTTTTTTTCAACATCAGTCTGATAAGCTA |
miR-23a | AUCACAUUGCCAGGGAUUUCCA | CATCACATTGCCAGGGAT | CGTCCAGTTTTTTTTTTTTTTTGGAA | CAGGTCCAGTTTTTTTTTTTTTTTGGAAATCCCTGGCAATGTGAT |
miR-23b | AUCACAUUGCCAGGGAUUACCAC | same as for miR-23a | TCCAGTTTTTTTTTTTTTTTGTGGTA | CAGGTCCAGTTTTTTTTTTTTTTTGTGGTAATCCCTGGCAATGTGAT |
miR-25 | CAUUGCACUUGUCUCGGUCUGA | CATTGCACTTGTCTCGGT | GGTCCAGTTTTTTTTTTTTTTTCAGA | |
miR-26a | UUCAAGUAAUCCAGGAUAGGCU | CGAGTTCAAGTAATCCAGGA | CCAGTTTTTTTTTTTTTTTAGCCTATC | CAGGTCCAGTTTTTTTTTTTTTTTAGCCTATCCTGGATTACTTGAA |
miR-27a | UUCACAGUGGCUAAGUUCCGC | CAGTTCACAGTGGCTAAGA | CAGTTTTTTTTTTTTTTTGCGGAA | CAGGTCCAGTTTTTTTTTTTTTTTGCGGAACTTAGCCACTGTGAA |
miR-101a | UACAGUACUGUGAUAACUGAA | CGCAGTACAGTACTGTGATAAC | AGGTCCAGTTTTTTTTTTTTTTTCAG | CAGGTCCAGTTTTTTTTTTTTTTTCAGTTATCACAGTACTGTA |
miR-103 | AGCAGCAUUGUACAGGGCUAUGA | AGAGCAGCATTGTACAGG | GGTCCAGTTTTTTTTTTTTTTTCATAG | |
miR-122 | UGGAGUGUGACAAUGGUGUUUGU | ACAGTGGAGTGTGACAATG | TCCAGTTTTTTTTTTTTTTTCAAACAC | CAGGTCCAGTTTTTTTTTTTTTTTACAAACACCATTGTCACACTCCA |
miR-125b | UCCCUGAGACCCUAACUUGUGA | CAGTCCCTGAGACCCTA | GTCCAGTTTTTTTTTTTTTTTCACAA | CAGGTCCAGTTTTTTTTTTTTTTTCACAAGTTAGGGTCTCAGGGA |
miR-139b-5p | UCUACAGUGCACGUGUCUCCAGU | TCTACAGTGCACGTGTCT | GTCCAGTTTTTTTTTTTTTTTACTGGA | CAGGTCCAGTTTTTTTTTTTTTTTACTGGAGACACGTGCACTGTAGA |
miR-150 | UCUCCCAACCCUUGUACCAGUG | GTCTCCCAACCCTTGTAC | GTCCAGTTTTTTTTTTTTTTTCACTG | CAGGTCCAGTTTTTTTTTTTTTTTCACTGGTACAAGGGTTGGGAGA |
miR-199b-3p | UACAGUAGUCUGCACAUUGGUU | CAGTACAGTAGTCTGCACAT | GTCCAGTTTTTTTTTTTTTTTAACCAA | CAGGTCCAGTTTTTTTTTTTTTTTAACCAATGTGCAGACTACTGTA |
miR-200b | UAAUACUGCCUGGUAAUGAUGA | ACAGTAATACTGCCTGGTAATG | GGTCCAGTTTTTTTTTTTTTTTCATC | CAGGTCCAGTTTTTTTTTTTTTTTCATCATTACCAGGCAGTATTA |
miR-200c | UAAUACUGCCGGGUAAUGAUGGA | AGTAATACTGCCGGGTAATG | GTCCAGTTTTTTTTTTTTTTTCCATC | CAGGTCCAGTTTTTTTTTTTTTTTCCATCATTACCCGGCAGTATTA |