Analysis of fatty acid composition in C. reinhardtii. (A) Separation by GC-FID of fatty acid methyl esters prepared from total lipids of C. reinhardtii cw15. (B) Comparison of the fatty acid composition of the TAG fraction and total lipids. The TAG fraction was isolated from cells cultivated in TAP-N for 2 days while total lipids were from cells grown in TAP medium. Values are mean of four independent experiments ± SD. Asterisks denote a statistically significant difference (t test, two-sided P < 0.05 at least).