Figure 5
Thermal stability analysis of the rCIα1 from maize, Pichia and human. (A) Western blot results of the maize-derived rCIα1 (CGB), rCIα1-OH (CGD), Pichia-derived rCIα1 (FE291), and rCIα1-OH (FE285) after 4°C incubation and pepsin treatment, using anti-foldon antibody. (B) Western blot results of the maize-derived rCIα1 (CGB), rCIα1-OH (CGD), and human CIα1 (HuC) after heat treatments under various temperatures and pepsin treatments as indicated, using both anti-foldon and anti-25 kD collagen antibody. M: molecular weight marker.