Figure 2

Analysis of electrophoretic mobility difference of rCIα1 in the CGB and CGD line. Equal volumes of total protein extracts from seeds of CGB, CGD (10 × concentrated by volume) and mixture of CGB+CGD extracts were loaded on the gel. The rCIα1 from CGB and CGD lines were detected by western blot using anti-foldon antibody. Pichia-derived rCIα1 (FE291) and rCIα1-OH (FE285) were included as controls and detected by Coomassie Brilliant Blue staining. Open arrows, rCIα1 from CGB or FE291; solid arrows, OH-rCIα1 from CGD or FE285. M, molecular weight marker.