Figure 6From: Facile, high efficiency immobilization of lipase enzyme on magnetic iron oxide nanoparticles via a biomimetic coatingThe effect of pH on the activity of free and immobilized lipase. The effect of pH on the activity of free (A) and immobilized (B) lipase. The samples were pre-incubated in phosphate buffer (10 mM) at the pH values and times indicated before determining enzyme activity at pH 7.0 and 37°C. The PD-MNPs were prepared using 2.5 mg/ml initial dopamine and lipase immobilization performed using 5 mg/ml initial lipase and 10 mg/ml PD-MNPs concentration.Back to article page