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Table 2 Purification of BmNPV-GFPuv-hPRR particles by Sephacryl S-1000 column chromatography with detergent-containing PBS

From: Purification of functional baculovirus particles from silkworm larval hemolymph and their use as nanoparticles for the detection of human prorenin receptor (PRR) binding

 

Volume (ml)

Titer (pfu ml-1)

Virus (pfu)

Recovery (%)

Hemolymph

7

2.38 × 108

1.67 × 109

100

Sephacryl S-1000 (PBS)

30

1.67 × 107

4.86 × 108

29

Hemolymph

7

8.30 × 107

5.81× 108

100

Sephacryl S-1000 (0.01% Triton X-100 in PBS)

30

1.66 × 107

4.98 × 108

86*

Hemolymph

7

3.33 × 108

2.33 × 108

100

Sephacryl S-1000 (0.1% Triton X-100 in PBS)

30

3.04 × 106

9.12 × 107

41

Hemolymph

7

2.38 × 108

1.67 × 109

100

Sephacryl S-1000 (0.01% Tween 20 in PBS)

30

2.29 × 107

6.87 × 108

41

  1. * This purification protocol was repeated several times and typical result is shown.
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BMC Biotechnology

ISSN: 1472-6750

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