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Table 2 Copy number of chloroplast (Cp) and mitochondrial (Mt) genomes in DNA isolated with the CTAB protocol (gDNA) or from isolated nuclei (nDNA)

From: Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing

Plant Nuclear genome size (Mb) DNA isolation protocol Organelle gDNA (count) nDNA (count) gDNA (%) nDNA (%)
Arabidopsis thaliana 150 A Cp 317 192 15 9.1
    Mt 3 6   
Brachypodium distachyon 300 B Cp 126 107 2.8 2.4
    Mt NA NA NA NA
Genlisea aurea 65 A Cp 5467 220   
    Mt 93 7   
Lemna gibba 447 B Cp 298 73 5.5 1.3
    Mt NA NA NA NA
Sorghum bicolor 692 B Cp 1043 578 5.3 2.9
    Mt 52 43   
Spirodela polyrhiza 120 B Cp 778 72   
    Mt NA NA NA NA
Vaccinium macrocarpon 450 C Cp 3568 695   
    Mt 785 35   
Zea mays 2300 B Cp 278 153 0.4 0.2
    Mt 16 6   
  1. DNA was isolated from Arabidopsis thaliana, Brachypodium distachyon, Genlisea aurea, Lemna gibba, Sorghum bicolor, Spirodela polyrhiza, Vaccinium macrocarpon and Zea mays. Chloroplast DNA contamination in DNA isolated from nuclei or with CTAB. % cpDNA is calculated by ([{# cpDNA copies/#copies of genomic gene}* cp genome size]/1C genome size)*100. Numbers assume a chloroplast and mitochondria genome size of 150 and 500 kb respectively. Data not available (NA).