Isolation and analysis of high quality nuclear DNA with reduced organellar DNA for plant genome sequencing and resequencing

Table 2 Copy number of chloroplast (Cp) and mitochondrial (Mt) genomes in DNA isolated with the CTAB protocol (gDNA) or from isolated nuclei (nDNA)

Plant	Nuclear genome size (Mb)	DNA isolation protocol	Organelle	gDNA (count)	nDNA (count)	gDNA (%)	nDNA (%)
Arabidopsis thaliana	150	A	Cp	317	192	15	9.1
			Mt	3	6
Brachypodium distachyon	300	B	Cp	126	107	2.8	2.4
			Mt	NA	NA	NA	NA
Genlisea aurea	65	A	Cp	5467	220
			Mt	93	7
Lemna gibba	447	B	Cp	298	73	5.5	1.3
			Mt	NA	NA	NA	NA
Sorghum bicolor	692	B	Cp	1043	578	5.3	2.9
			Mt	52	43
Spirodela polyrhiza	120	B	Cp	778	72
			Mt	NA	NA	NA	NA
Vaccinium macrocarpon	450	C	Cp	3568	695
			Mt	785	35
Zea mays	2300	B	Cp	278	153	0.4	0.2
			Mt	16	6

DNA was isolated from Arabidopsis thaliana, Brachypodium distachyon, Genlisea aurea, Lemna gibba, Sorghum bicolor, Spirodela polyrhiza, Vaccinium macrocarpon and Zea mays. Chloroplast DNA contamination in DNA isolated from nuclei or with CTAB. % cpDNA is calculated by ([{# cpDNA copies/#copies of genomic gene}* cp genome size]/1C genome size)*100. Numbers assume a chloroplast and mitochondria genome size of 150 and 500 kb respectively. Data not available (NA).

ISSN: 1472-6750