Determination of some significant batch culture conditions affecting acetyl-xylan esterase production by Penicillium notatum NRRL-1249

Table 1 Application of 2-factorial design at various process parameters for AXE production by P. notatum NRRL-1249*

Process parameters at 2-factorial design					AXE production (U/g)
Wheat bran (g)^A	MSS pH^B	Time of incubation (h)^C	Urea (%, w/w)^D	Tween-80 (%, v/w)^E	Observed	Predicted
5	4.5	24	na	na	185	198
7.5	4.5	36	na	na	312	385
10	5	36	0.1	0.25	455	560
10	5	48	0.2	0.5	760	832
12.5	5.5	60	0.3	0.75	646	811

*The different letters represent significant process parameters for AXE production. Statistical analysis of the model was based on 2-factorial experimental design. 'na' means that nitrogen source was not added.

ISSN: 1472-6750