Figure 6
From: Identification of a novel temperature sensitive promoter in cho cells
Temperature-shift induced change in gene expression. Two representative promoter constructs, the Sps1 full-length and the dS18 core promoter sequence, were analyzed for relative mRNA levels (qRT) and luciferase protein expression (Luc). Analyses were performed at two different temperatures at 33°C and 37°C measured after 24 hours. The shown values represent the mRNA and protein ratios at 33°C relative to 37°C (y-axis) normalized to pGL3 control vector.