Figure 4
From: Increasing gene dosage greatly enhances recombinant expression of aquaporins in Pichia pastoris
Recombinant expression levels of aquaporin isoforms in single copy clones. Western blot with crude cell extracts from single copy representatives of the four different aquaporins (top). The calculated aquaporin copy number ± SD of each clone is also included (bottom). The AtSIP1;1 isoform is poorly expressed as compared with the other isoforms and the very faint monomeric band for one of the clones is indicated by an arrow.