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Figure 4 | BMC Biotechnology

Figure 4

From: Efficient production of human acidic fibroblast growth factor in pea (Pisum sativum L.) plants by agroinfection of germinated seeds

Figure 4

Western blot of the total soluble protein extracts from plants agroinfected with both GV3101 carrying pCAE1 and GV3101 carrying pCAE2-GFP. (a and c) Western blot probed with antibody against RUBISCO large subunit as control for the amount of proteins loaded. (b) Proteins probed with anti-GFP polyclonal antibody. Both for a and b: Lane 1, low-molecular marker; lanes 2 and 3, purified GFP proteins, lane 4, proteins from bacteria; lane 5, proteins from non-inoculated pea plants; lane 6, proteins from plants agroinfected by leaf injection, lane 7, proteins from plants agroinfected by vacuum infiltration. (d) Western blot of the total soluble protein extracts from the plants agroinfected with GV3101-pCAE1 and GV3101-aFGF probed with anti-aFGF antibody. Both for c and d: lane 1, low-molecular marker; lane 2, commercial aFGF; lane 3, proteins from bacteria; lane 4, proteins from non-inoculated pea plants; lane 5, proteins from plants agroinfected by leaf injection; lane 6, proteins from plants agroinfected by vacuum infiltration.

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BMC Biotechnology

ISSN: 1472-6750

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