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Table 2 PCR mixes used and their composition

From: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

  PCR mixes*
Component** I II III IV
Tris-HCl (mM) [pH]*** 10 [8.0] 75 [8.8] 10 [ 8.0] 20 [8.8]
KCl (mM) 50 - 50 10
(NH4)2SO4 (mM) - 20 - 10
MgSO4 (mM) - - - 2
Triton X-100 (%) 0.1 - 0.1 0.1
Tween 20 (%) - 0.01 - -
MgCl2 mM 2.5 2.5 2.5 -
DMSO (%) - - 5 -
dNTPs μM 200 200 200 200
Taq DNA pol. (U/ml) 25 25 25 25
anti-Taq mAb (nM) 22 22 22 22
  1. * Final concentrations of the component during PCR.
  2. ** Components present at the same concentrations in all mixes include 200 μM dNTPs, Taq DNA pol. (25 U/ml) and anti-Taq mAb (22 nM). Oligonucleotide primers, DNA templates, DNA dyes and additives/enhancers were added immediately before the assay.
  3. *** pH at 21°C.