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Table 2 PCR mixes used and their composition

From: 1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

 

PCR mixes*

Component**

I

II

III

IV

Tris-HCl (mM) [pH]***

10 [8.0]

75 [8.8]

10 [ 8.0]

20 [8.8]

KCl (mM)

50

-

50

10

(NH4)2SO4 (mM)

-

20

-

10

MgSO4 (mM)

-

-

-

2

Triton X-100 (%)

0.1

-

0.1

0.1

Tween 20 (%)

-

0.01

-

-

MgCl2 mM

2.5

2.5

2.5

-

DMSO (%)

-

-

5

-

dNTPs μM

200

200

200

200

Taq DNA pol. (U/ml)

25

25

25

25

anti-Taq mAb (nM)

22

22

22

22

  1. * Final concentrations of the component during PCR.
  2. ** Components present at the same concentrations in all mixes include 200 μM dNTPs, Taq DNA pol. (25 U/ml) and anti-Taq mAb (22 nM). Oligonucleotide primers, DNA templates, DNA dyes and additives/enhancers were added immediately before the assay.
  3. *** pH at 21°C.
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BMC Biotechnology

ISSN: 1472-6750

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