1,2-propanediol-trehalose mixture as a potent quantitative real-time PCR enhancer

BMC Biotechnology

Table 1 DNA dyes, their origin and properties

DNA dye	Origin	Stock concentration	Final concentration	Absorption maximum	Emission maximum
SGI	Invitrogen	10 mM in DMSO*	0.33 μM	497	520
SYTO-9	Invitrogen	5 mM in DMSO	2 μM	485	498
SYTO-13	Invitrogen	5 mM in DMSO	2 μM	488	509
SYTO-82	Invitrogen	5 mM in DMSO	2 μM	541	560
EvaGreen	Biotium	25 mM in DMSO	1.33 μM	500	530
LCGreen	Idaho	10 × concentrated	1×	440-470	470-520
ResoLight	Roche	20 × concentrated	1×	450-500	487

ISSN: 1472-6750