Figure 3

Yield, extent of liposome association and solubility of each protein. (A) Yield of MPs. MPs were synthesized using the wheat cell-free system supplemented with [¹⁴C]-leucine and liposomes. Proteins were precipitated by 10% trichloroacetic acid and radioactivity was measured by liquid scintillation counter and the yield of each protein was calculated. The numbers on the x-axis correspond to the proteins in Table I. (B) Yield of MPs in each protein family. (C) Amount of liposome-associated proteins. MPs were synthesized using the wheat cell-free system supplemented with [¹⁴C]-leucine and liposomes. After synthesis, the translation reaction was separated by sucrose DGU and fractions were collected from the top of the tube. The protein content of each fraction was estimated through radioactivity measurements of proteins precipitated by 10% trichloroacetic acid. The extent of association was calculated by combining the liposome fractions six through eight. For each protein, the specific amount of association with liposomes was calculated from the synthesis yield and extent of association. (D) The relationship between the extent of association and solubility of each protein. Membrane and soluble proteins were synthesized using the wheat cell-free system supplemented with or without liposomes and [¹⁴C]-leucine. Proteins synthesized without liposomes were separated into soluble and insoluble fraction by centrifugation. The unprocessed synthesis reactions and the insoluble fractions were precipitated by 10% trichloroacetic acid and radioactivity was measured by liquid scintillation counter to determine the solubility for each protein. The extent of association for soluble proteins was calculated using the same method as for MPs. The extent of association and solubility of membrane and soluble proteins are plotted. (E) Relationship between extent of association and yield for each protein. Values for the extent of association and yield of each protein are plotted.