Figure 2
Association of synthesized MPs with liposomes. (A) SDS-PAGE analysis of liposome association of MPs. MPs were synthesized using a wheat cell-free system supplemented with liposomes and [14C]-leucine. After the synthesis reaction, the reaction mixture was subjected to sucrose DGU and fractions were collected from the top of the tube. Synthesized proteins in each fraction were detected by SDS-PAGE and autoradiography. The number of TMDs of each protein is shown in parentheses. T means total fraction of reaction mixture. (B) Yield of synthesized proteins in each fraction. After fractionation, synthesized proteins were precipitated by 10% trichloroacetic acid and radioactivity of each fraction was measured by liquid scintillation counter.