Effect of timing of liposome-supplementation on transport activity of synthesized AtPPT1. Three types of AtPPT1 proteins were prepared, 1: AtPPT1 synthesized with liposomes, 2: AtPPT1 synthesized without liposomes, 3: AtPPT1 synthesized without liposomes and mixed with liposomes post-synthesis. Each type of synthesis was reconstituted in liposomes that had been pre-loaded with 30 mM phosphate. Uptake of [32P] phosphate into the liposomes was measured. The 100% exchange activity of the synthesized protein was 151 n mol/min/mg proteins. Data is reported as the mean±SD of values from three independent experiments.