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Table 2 Amino acid sequences of zebrafish wild-type (WT) and mutated Aqp3b

From: Design and characterization of genetically engineered zebrafish aquaporin-3 mutants highly permeable to the cryoprotectant ethylene glycol

Construct Loop A Loop B Loop C Loop D Loop E
DrAqp3b-WT HILSGGSHGMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
H53A HILSGGSAGMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
G54A HILSGGSHAMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
H53A/G54H HILSGGSAHMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
H53A/G54H/T85A HILSGGSAHMFLTV SGGHINPAVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
T85A HILSGGSHGMFLTV SGGHINPAVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
R95A HILSGGSHGMFLTV SGGHINPTVTFSLCLLGAEPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
E96A HILSGGSHGMFLTV SGGHINPTVTFSLCLLGRAPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
R99A HILSGGSHGMFLTV SGGHINPTVTFSLCLLGREPWAKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
H154A HILSGGSHGMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKALTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
G54H/H154A HILSGGSHHMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKALTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
H53A/H154A HILSGGSAGMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKALTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
H53A/G54H/H154A HILSGGSAHMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKALTLL IVDPYNNPIPQGLEA AVNPARDLGPRIFTAIAG
A217T HILSGGSHGMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPTRDLGPRIFTAIAG
T85A/A217T HILSGGSHGMFLTV SGGHINPAVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPYNNPIPQGLEA AVNPTRDLGPRIFTAIAG
Y182A HILSGGSHGMFLTV SGGHINPTVTFSLCLLGREPWRKFP FATYPSKHLTLL IVDPANNPIPQGLEA AVNPARDLGPRIFTAIAG
  1. Amino acid sequence of the five loops of WT and DrAqp3b mutants. The residues mutated and the substitutions are underlined in bold.