Table 2 Amino acid sequences of zebrafish wild-type (WT) and mutated Aqp3b
Construct | Loop A | Loop B | Loop C | Loop D | Loop E |
|---|---|---|---|---|---|
DrAqp3b-WT | HILSGGSHGMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
H53A | HILSGGSAGMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
G54A | HILSGGSHAMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
H53A/G54H | HILSGGSAHMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
H53A/G54H/T85A | HILSGGSAHMFLTV | SGGHINPAVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
T85A | HILSGGSHGMFLTV | SGGHINPAVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
R95A | HILSGGSHGMFLTV | SGGHINPTVTFSLCLLGAEPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
E96A | HILSGGSHGMFLTV | SGGHINPTVTFSLCLLGRAPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
R99A | HILSGGSHGMFLTV | SGGHINPTVTFSLCLLGREPWAKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
H154A | HILSGGSHGMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKALTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
G54H/H154A | HILSGGSHHMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKALTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
H53A/H154A | HILSGGSAGMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKALTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
H53A/G54H/H154A | HILSGGSAHMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKALTLL | IVDPYNNPIPQGLEA | AVNPARDLGPRIFTAIAG |
A217T | HILSGGSHGMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPTRDLGPRIFTAIAG |
T85A/A217T | HILSGGSHGMFLTV | SGGHINPAVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPYNNPIPQGLEA | AVNPTRDLGPRIFTAIAG |
Y182A | HILSGGSHGMFLTV | SGGHINPTVTFSLCLLGREPWRKFP | FATYPSKHLTLL | IVDPANNPIPQGLEA | AVNPARDLGPRIFTAIAG |