Comparison of expression, purification and characterization of a new pectate lyase from Phytophthora capsiciusing two different methods

Table 2 Purification summary of PCPEL2 expressed by pMAL system

Purification steps	Total protein (mg)	Yield (%)	Total activity^b (U)	Specific activity (U/mg protein)	Purification (fold)
Crude supernatant^a	860.5	100	13768	16	1
Amylose Resin affinity chromatography with MBP	22.6	2.63	3051	135	8.4
Amylose Resin affinity chromatography without MBP	7.5	0.87	6450	860	53.8

^a The starting material was 1 L of crude E. coli supernatant.
^b Enzyme activity was defined as the amount of enzyme that produced a change in absorbance of 0.001 at 232 nm/min, as determined by the formation of unsaturated uronide.

ISSN: 1472-6750