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Figure 2 | BMC Biotechnology

Figure 2

From: Rapid obtention of stable, bioluminescent tumor cell lines using a tCD2-luciferase chimeric construct

Figure 2

Western blot analysis of luciferase 2 (luc2) and tCD2-luc 2 proteins in short-term transfected 293 cells. The 293 cells were seeded and transfected as explained in the legend of Figure 1. They were grown in situ for 48 h prior to protein extraction and western blot analysis. The wild-type luciferase 2 and the tCD2-luc2 chimera are pointed out by an arrowhead and a star, respectively. An apparent cleaved fragment of the tCD2-luc2 protein with a size identical to the wild-type luciferase 2 is pointed out by a dash. The western blot membranes were restained with an anti-actin (Sigma) for loading control.

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