Figure 7
From: Identification of gliadin-binding peptides by phage display
Concentration dependency of peptide gliadin/anti-gliadin antibody binding inhibition. Gliadin was diluted to 1 ng/ml and mixed with 1.67 nmoles, 0.167 pmoles, and 0.0167 fmoles (1015, 109, and 105 molecules respectively) of peptides P61, P64, and CP31. Afterwards the gliadin/peptides mixes were added to anti-gliadin, monoclonal antibody-coated wells and incubated for 1 h. The formed gliadin/antibody complexes immobilised in the wells were quantified as described (see Methods) and given as absorbance units at 450 nm (Y-axis).