Figure 5

Binding of synthetic peptides to gliadin proteins. Microtiter wells were coated with increasing amounts of gliadin (ng) as indicated on the X-axis. Afterwards 10¹⁵ molecules (1.67 nmoles) of biotinylated peptides, P61, P64, or P22 were added to the wells. Bound peptides were quantified as absorbance units at 405 nm (OD units) by means of the biotin adduct as described in Methods. P61/64 is a 1:1 mixture of peptides P61 and P64.