Figure 4
From: Identification of gliadin-binding peptides by phage display
Gliadin-anti-gliadin antibody interaction in the presence of peptide-displaying phage. The Y-axis shows different phage displaying peptides tested. Phage (1011 pfu/ml) were added to microtiter wells coated with 10 ng gliadin. After incubation and extensive washing, pooled sera from patients with suspected CD were added. The amount of bound antibody was determined by adding a secondary AP-tagged goat anti-human IgG antibody, and measured as absorbance at 405 nm (Y-axis).