Figure 3

TEM images of aggregates grown from αS variants labelled with Alexa Fluor 488. The scale bar is 200 nm wide and all images are shown at the same magnification. All samples contained 10 mM pH 7.5 NaPhos, and unless otherwise specified, the solutions were incubated at 37°C with shaking. The protein concentrations, incubation times t, and other solution conditions are as follows: (A) 43 μM WT/E130C-Al488 αS with 150 mM NaCl, t = 1 week. (B) 43 μM WT/S9C-Al488 αS with 150 mM NaCl and 5% TFE, t = 1 week. (C) 50 μM A30P/E130C-Al488 αS with 150 mM NaCl, t = 2 weeks. (D) 50 μM A53T/S9C-Al488 αS with 150 mM NaCl, t = 2 weeks. (E) 50 μM A30P/E130C-Al488 αS with 150 mM NaCl and 5% TFE, t = 2 weeks. (F-G) 50 μM A30P/E130C-Al488 αS with 5% TFE, t = 2 weeks. (H) 43 μM WT/S9C-Al488 αS with150 mM NaCl and 15% TFE, incubated at 22°C under quiescent conditions, t = 5 weeks. (I) 50 μM WT/E130C-Al488 αS with 150 mM NaCl and 5% TFE, incubated at 22°C under quiescent conditions, t = 2 weeks. (J) 50 μM WT/E130C-Al488 αS with 5% TFE, incubated at 22°C under quiescent conditions, t = 1 week. (K) 50 μM A30P/S9C-Al488 αS with 10% TFE, t = 2 weeks. (L) 50 μM A30P/E130C-Al488 αS, t = 2 weeks.