Figure 2
From: Expression of recombinant multi-coloured fluorescent antibodies in gor -/trxB- E. colicytoplasm
The assembly of the 4D5 recombinant antibody constructs. (A) 4D5-8 scFv 15 amino acid linker. The mature protein begins with VH chain, followed by a (Gly4Ser)3 linker, VLchain. The sites used to directionally insert the in-frame scFv encoding sequence are underlined (NcoI-NotI). (B) 4D5-8 scFv 5 amino acid linker. The mature protein begins with VH chain, followed by a (Gly4Ser), VL chain. The sites used to directionally insert the in-frame scFv encoding sequence are underlined (NcoI-NotI). The linker terminal Gly Ser underlined is encoded by an in frame BamHI site. (C) Modified mRFP1. The mRFP1 sequence is flanked by two in-frame BamHI sites underlined. The alternative BFP, CER and CIT encoding sequences were modified in a similar manner. (D) 4D5-8RFP. The modified mRFP1 (C) was inserted into 4D5-8 scFv 5 amino acid linker construct (B) at the BamHI site.