Table 1 Effect of transfection conditions on transfection efficiency, cumulative rFVIII production, and cell specific rFVIII production (qFVIII) rate by transient transfection of HEK293 suspension cultures
Condition | Transfection efficiency (%) | *Cumulative active rFVIII production (IU) | qFVIII (ng FVIII/106 cell.h) |
|---|---|---|---|
0.4 μg DNA/10 6 cel | 51.1 | 25.4 | 0.24 |
0.6 μg DNA/10 6 cel | 50.0 | 35.1 | 0.30 |
0.8 μg DNA/10 6 cel | 55.9 | 29.6 | 0.21 |
0.4 μg DNA/10 6 cel 37°C Repeated Transfection | 57.3 | 21.9 | 0.43 |
0.4 μg DNA/10 6 cel 34°C Repeated Transfection | 52.8 | 49.3 | 0.58 |