Table 1 Effects of culture factors on cell growth and reduction activity of Acetobacter sp. CCTCC M209061
Factor | Biomass (g/L) | Specific activity (μmol/min/g) | e.e. (%) |
|---|---|---|---|
Extra vitamins | |||
Control | 0.94 ± 0.04 | 34.15 ± 1.05 | ≥ 99 |
Vitamin B1 | 0.93 ± 0.03 | 34.36 ± 0.94 | ≥ 99 |
Nicotinic acid | 0.93 ± 0.01 | 35.04 ± 1.07 | ≥ 99 |
Pyridoxine | 0.95 ± 0.02 | 34.18 ± 0.89 | ≥ 99 |
Ascorbic acid | 0.94 ± 0.03 | 33.88 ± 0.93 | ≥ 99 |
Biotin | 0.93 ± 0.04 | 34.59 ± 0.86 | ≥ 99 |
pH | |||
4 | 0.38 ± 0.01 | 11.61 ± 0.39 | ≥ 99 |
5 | 0.95 ± 0.02 | 34.67 ± 1.10 | ≥ 99 |
6 | 0.94 ± 0.04 | 34.15 ± 1.05 | ≥ 99 |
7 | 0.82 ± 0.03 | 29.37 ± 0.84 | ≥ 99 |
8 | 0.48 ± 0.01 | 23.03 ± 0.58 | ≥ 99 |
Temperature (°C) | |||
25 | 0.79 ± 0.03 | 33.01 ± 1.10 | ≥ 99 |
30 | 0.95 ± 0.02 | 34.67 ± 1.10 | ≥ 99 |
35 | 0.86 ± 0.02 | 31.38 ± 1.05 | ≥ 99 |
40 | 0.62 ± 0.02 | 23.21 ± 0.61 | ≥ 99 |
45 | 0.08 ± 0.01 | 0 | - |
Shaking rate (rpm) | |||
40 | 0.76 ± 0.02 | 39.55 ± 1.12 | ≥ 99 |
80 | 0.96 ± 0.02 | 36.76 ± 1.03 | ≥ 99 |
100 | 0.95 ± 0.02 | 34.67 ± 1.10 | ≥ 99 |
120 | 0.97 ± 0.02 | 30.14 ± 0.99 | ≥ 99 |
160 | 0.96 ± 0.03 | 24.24 ± 0.65 | ≥ 99 |
200 | 0.97 ± 0.04 | 17.74 ± 0.47 | ≥ 99 |