Figure 7
From: A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors
Characterization of the optimized biosensor. (A-B) Emission scans of H3K27-MetBio2 (A) and H3K27-MetBio3 (B) after treatment with vSET in the presence or absence of SAM, normalized to acceptor intensity. (C) Time course of FRET ratio changes for H3K27-MetBio variants treated with vSET and SAM and calculated using the ratio of fluorescence intensities at 526 nm and 494 nm.