Figure 4
From: A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors
Structure and characterization of H3K27-MetBio1. (A) Sequence alignment of H3K27-MetBio1, H3K27-MetBio2, and H3K27-MetBio3. Underlined residues in the H3K27-MetBio2 and H3K27-MetBio3 sequences denote amino acids encoded by restriction sites for Kpn1 (GT), Sal1 (GST), and Eag1 (TAG). (B) Emission spectra of the H3K27-MetBio1 protein in both the methylated and unmethylated state, normalized to acceptor intensity. (C) Image-based emission ratios for H3K27-MetBio1 in colonies of E. coli that were grown on different plate compositions. All plates contained 1 mM IPTG.