Figure 3
From: A bacteria colony-based screen for optimal linker combinations in genetically encoded biosensors
Optimization of expression conditions. Western blots showing expression of vSET for colonies of E. coli grown in the presence or absence of inducer. For each condition, 20 μg of total protein was loaded in the left lane and 2 μg was loaded in the right lane. (A) Media containing IPTG (1 mM) and L-arabinose (13 mM). (B) Media containing IPTG (1 mM) and D-glucose (11 mM). (C) Media conditions identical to (B), 3 h after being sprayed with a concentrated solution of L-arabinose. (D) Media containing IPTG (1 mM).