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Figure 2 | BMC Biotechnology

Figure 2

From: Selective targeted delivery of the TNF-alpha receptor p75 and uteroglobin to the vasculature of inflamed tissues: a preliminary report

Figure 2

In vitro L19-hUG-TNFRII biological activity. A) Inhibitory activity of TNF-alpha cytotoxicity by different concentrations of L19-UG-TNFRII using LM mouse fibroblasts treated with 2 pM TNF-alpha. B) L19-UG-TNFRII bound to ED-B is able to inhibit TNF-alpha cytotoxicity (in situ inhibition). The cytotoxic activity of 25 pM TNF-alpha was evaluated on LM cells using plates pre-coated with the recombinant FN fragment 7.ED-B.8.9 and pre-incubated with different concentrations of L19-UG-TNFRII (5-3125 pM). To assess the role of the L19 component the experiment was also carried out mixing the L19-UG-TNFRII with a large excess of ED-B to inhibit of the scFv L19 moiety. After washing out the unbound fusion protein, TNF-alpha was inhibited only by L19-UG-TNFRII bound to the recombinant FN fragment with which the plates were coated. The means ± S.D. are reported.

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BMC Biotechnology

ISSN: 1472-6750

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