Figure 5

Total secreted Cellulase in Lactose induced cultures. Cellulase secretion per gram dry weight in T. reesei strains QM 6a, JKTR2-6 and cre1 deletion, 12, 24 and 36 hours post transfer. Mycelia for inoculation were harvested after growth for 24 hours in 1% (w/v) glucose medium. Mycelia were washed with liquid carbon free medium and 200 mg was added to 50 ml culture medium in 250 ml Erlenmeyer flasks. Cultures were grown at 30°C, shaken at 200 RPM, and were harvested 12 hours, 24 hours and 36 hours post inoculation. Total secreted cellulase activity was measured using the EnzChek Cellulase Substrate expressed as fluorescence per mg. Dry weights are shown in Additional File 1 and levels of total cellulase activity are shown in Additional File 3. Glucose concentrations were determined by HPLC analysis. The carbon sources for each of the cultures are a) 2% glucose, b) 2% glucose and 2% lactose, c) 2% lactose, d) 2% sorbitol and e) 2% glycerol. The strain key is f). The data presented is based on biological triplicates that were assayed in duplicate.