Table 2 Sequencing primers used for sequence verification and repeat number determination
From: Authentication of African green monkey cell lines using human short tandem repeat markers
STR Marker | Sequencing Primers (5'-3') | Amplicon Size (bp) | Tm (°C) |
|---|---|---|---|
D8S1106 | F- GTTTACCCCTGCATCACTGG R- GGTGGCCTAACCAGAGTTGA* | 267-291 | 55 |
DYS389 | F - CCAACTCTCATCTGTATTATCTATG R - GTCTTATCTCCACCCACCAGA | 321-337 | 60 |
D1S518 | F - GCAGATCTTGGGACTTCTCG R - GTGTGGGCAACTGCATTAGAG | 182-198 | 55 |
D6S1017 | F - CTGGCACAGGATAGGTGCTC R - GATTGAACCAGATGGGAACGA | 354-374 | 59 |
D17S1304 | F - ACCATGTCCTCTGGTTCTGG R - GTTTCTTACAGGTGGGACTTGGTGAAA | 197-213 | 60 |
D4S2408 | F - TCATTTCCATAGGGTAAGTGAAAA R - GCCATGGGGATAAAATCAGA | 336-360 | 60 |
D5S1467 | F - GCCTAAGGTGGTGAATTGGA R - GAAGATGGCCCATTTCCATTT* | 313-329 | 60 |
D19S245 | F - GACCTGCAATCAGCCATTTT R - GTTCTTGCAGTCTGTGGCTTG | 225-249 | 60 |