New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme

Table 1 Purification of NADH oxidase from E.coli.

Entry^a	Protein (mg/ml)	Specific Activity (U/mg)^b	Purification factor	Purification yield (%)
1	26.29	0.25	1	100
2	6.48	1.88	7.5	100
3	3.3	3.69	14.7	80
4	2.93	4.15	16.6	70

^aEntry 1: Crude extract. Entry 2: Heat treatment (80°C) for 45 min. Entry 3: Heat treatment (80°C 45 minutes) and further incubation with PEI-ag for 30 min. Entry 4: Heat treatment (80°C 45 minutes) and sequential ^b activity at 65°C, pH 7 without exogenous FAD/FMN.

ISSN: 1472-6750