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Table 1 Purification of NADH oxidase from E.coli.

From: New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme

Entrya Protein (mg/ml) Specific Activity (U/mg)b Purification factor Purification yield (%)
1 26.29 0.25 1 100
2 6.48 1.88 7.5 100
3 3.3 3.69 14.7 80
4 2.93 4.15 16.6 70
  1. aEntry 1: Crude extract. Entry 2: Heat treatment (80°C) for 45 min. Entry 3: Heat treatment (80°C 45 minutes) and further incubation with PEI-ag for 30 min. Entry 4: Heat treatment (80°C 45 minutes) and sequential b activity at 65°C, pH 7 without exogenous FAD/FMN.