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Figure 1 | BMC Biotechnology

Figure 1

From: New biotechnological perspectives of a NADH oxidase variant from Thermus thermophilus HB27 as NAD+-recycling enzyme

Figure 1

Effect of exogenous flavin cofactor on the activities of recombinant NOX in soluble form. Before purification, crude extracts were incubated with different flavin nucleotides at different concentrations (from 0 to 150 μM of either FMN or FAD). Activity was determined in 50 mM sodium phosphate at pH 7 and 37°C. Soluble enzyme with FAD (triangles), soluble enzyme with FMN (circles). The relative activity was calculated taking as fraction 1 the initial observed activity without flavin cofactor.

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BMC Biotechnology

ISSN: 1472-6750

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