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Figure 5 | BMC Biotechnology

Figure 5

From: Generation of recombinant single-chain antibodies neutralizing the cytolytic activity of vaginolysin, the main virulence factor of Gardnerella vaginalis

Figure 5

Stability of anti-VLY scFvs during long-term storage. (A) Analysis of anti-VLY scFvs stored at the concentration of 0.65 mg/ml for 12 months by SDS-PAGE under reducing conditions. Lane 1 - 9B4-derived scFv (variant VH-VL); lane 2 - 23A2-derived scFv (variant VH-VL). Lane M - pre-stained protein molecular weight marker (Thermo Scientific Fermentas). (B) Testing of anti-VLY scFvs stored at the concentration of 0.65 mg/ml for 1, 6 and 12 months for the ability to neutralize VLY-mediated lysis of human erythrocytes.

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BMC Biotechnology

ISSN: 1472-6750

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