Figure 4
TGFαL3SEA D227A inhibits tumour growth in vivo. (A) Thirty C57BL/6 mice were inoculated subcutaneously with 1.0 × 106 B16 melanoma cells. When the tumour length exceeded 0.5 cm, the mice were separated into equal-size groups and injected intraperitoneally with 6 pmol (asterisk), 60 pmol (multiplication), 600 pmol TGFαL3SEAD227A (black triangle), 60 pmol rSEA(black square) or PBS (black diamond), respectively, four times at one day intervals. The tumour sizes were measured with callipers at the time points indicated. The tumour volumes were calculated as (length × width2)/2. Each point shows the mean ± SD of the tumour volume from ten treated mice. (B) Twenty mice were inoculated with 1.0 × 106 B16 melanoma cells each and then randomised into two groups. The mice were treated with 60 pmol TGFαL3SEAD227A (black diamond) or PBS (black square) according to the protocol above when the tumour length exceeded 1 cm. The survival percentages were analysed by Kaplan Meier Plot.