Figure 3

Assessment of RNA degradation and RNA performance in RT-PCR by the multiplex endpoint RT-PCR assay. The sizes of the molecular weight markers (MW) are given on the left, whereas the sizes of the TBP amplicons are indicated on the right. Sizes are given in base pairs. Lane 1 and 10 were loaded with the no template control (NTC). Lane 2 and 9 were loaded with the PCR reaction obtained from a cDNA mixture synthesised from RNA extracted from different cell lines and serves as positive control, showing all four PCR amplification products of the expected size. Lane 8 was loaded with the PCR reaction obtained from genomic DNA and served as negative control. Lanes 3 to 7 were loaded with RT-PCR reactions obtained from cDNAs synthesised from total RNA derived from needle microdissected FFPE breast tumour tissues (Lane 3: P1-98-05, replicate 1; Lane 4: P1-98-06, replicate 1; Lane 5: P1-98-07, replicate 1; Lane 6: P1-98-08, replicate 1; Lane 7: P1-98-09, replicate 1).