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Figure 8 | BMC Biotechnology

Figure 8

From: An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

Figure 8

Reactivity of generated monoclonal antibodies with the endogenous P. falciparum proteins. Fluorescence staining (column 2, 3 & 4) and DIC micrographs (column 1) of P. falciparum parasites. While anti-PFF0620C mAb specifically stained salivary gland sporozoites (line 5), anti-PF14_0325 mAb (line 3) and anti-PFD1130W mAb (line 2) specifically reacted with schizont blood stage parasites. Staining with only the Cy3-labelled rabbit anti-mouse IgG secondary antibody served as negative control (line 1 & 4). Parasite nuclei were stained with DAPI.

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BMC Biotechnology

ISSN: 1472-6750

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