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Figure 6 | BMC Biotechnology

Figure 6

From: An efficient system to generate monoclonal antibodies against membrane-associated proteins by immunisation with antigen-expressing mammalian cells

Figure 6

Immunofluorescence microscopic screening of hybridoma cell culture supernatants for antibodies binding to cell-surface expressed PFF0620C. PFF0620C-HEK cells (right column) and untransfected HEK cells (left column) were fixed with methanol and stained with hybridoma supernatants of individual cell culture wells taken 15 days after the fusion. A FITC-labelled anti-mouse IgG antibody served as secondary antibody. Representative fluorescence micrographs from the PFF0620C-fusion are shown, demonstrating typical results for hybidoma lines producing PFF0620C-specific (line 1, 2 & 3), non-binding (line 4), or HEK cell antigen specific (line 5 & 6) antibodies. For some wells mixed specificity was seen, indicating presence of two ore more cell clones with one producing Abs specific for the transgene and the other producing Abs specific for a HEK-cell protein (line 7).

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BMC Biotechnology

ISSN: 1472-6750

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