Mimotopes selected out with U001 were input and aligned with ClustalW. (A) Alignment of multiple sequences by ClustalW. (B) The frequency of a given amino acid at each position of the alignment was calculated and displayed in a table. (C) A 3D bar figure was created. The x axis represents the 20 amino acid types and gap, whereas the y axis is the frequency of occurrence and the z axis is the position of the aligned sequences. (D) The alignment could be managed with JalView.