Figure 4
From: Improved Tet-responsive promoters with minimized background expression
Dose response analyses of Ptet-1 and Ptet-T6 in transduced HeLa-EM2 cell populations. (A) Determination of dose response via eGFP function of lmg*. Mean fluorescence units (mfu) are given for the complete populations (≥ 95% purity). For value ranges see insets in "C". (B) The simultaneous analysis of identical populations for specific luciferase activity of lmg*. The rlu values ranged from about 4.5 × 104 (± 2.8 × 103) to 1.6 × 107 (± 4.7 × 105) rlu/μg for Ptet-1 and 2.9 × 103 (± 1.1 × 102) to 2.3 × 107 (± 3.9 × 105) rlu/μg for Ptet-T6 in the off-and on-state of the system. (C) FACS-analysis of a representative cell population for Ptet-1 and Ptet-T6 promoters transferred by the viral vectors. Mfu values of the whole population were given as inset. The cellular background of the GFP-negative Hela-EM2 parental cell line was 1.83. The Dox concentrations used are indicated. Induction was for 96 hours. Results shown in figs. A and B were obtained from two independently generated populations for each vector.